Protease Inhibitor Cocktail (100x strength) from Cytoskeleton, Inc.

Product Specs
ItemProtease Inhibitor Cocktail (100x strength)
CompanyCytoskeleton, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberPIC02
TargetPepstatin A, Leupeptin, Benzamidine, Na-p-tosyl-L-arginine methyl ester (TAME)
Quantity1 x 1 ml
Brand/TrademarkCytoskeleton, Inc

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Cytoskeleton, Inc.

1830 S. Acoma St.

Denver, Colorado 80223

United States

Phone: 303 322-2254
303 322-2257

Company Profile

Website: http://cytoskeleton.com/

(1) The Cytoplasmic Region of SARAF Reduces Triple-Negative Breast Cancer Metastasis through the Regulation of Store-Operated Calcium EntryInternational Journal of Molecular SciencesMarch 10, 2023María Paz Saldías, Pablo Cruz, Ian Silva, Octavio Orellana-Serradell, Boris Lavanderos, Diego Maureira, Raquel Pinto, Oscar CerdaMO, USA, Cat#78830) and a protease inhibitor cocktail (PIC; Cytoskeleton, Inc, Denver, CO, USA, Cat#PIC02), adjusted to pH 7.4 for 30 min at 4 °C. The lysates were centrifuged at 12,000× g at 4 °C for 10 min.

(2) Spatial regulation of the glycocalyx component podocalyxin is a switch for prometastatic functionScience AdvancesFebruary 3, 2023Alvaro Román-Fernández, Mohammed A. Mansour, Fernanda G. Kugeratski, Jayanthi Anand, Emma Sandilands, Laura Galbraith, Kai Rakovic, Eva C. Freckmann, Erin M. Cumming, Ji Park, et al.ubiquitin-Trap (UB-Trap), BlastR Lysis Buffer (# BLST01, Cytoskeleton) with 1× protease inhibitor cocktail (PIC02, Cytoskeleton) and 1× deubiquitination/small ubiquitin-like modifier (SUMOylation) inhibitor (N-ethylmaleimide

(3) Carboxypeptidase E Independently Changes Microtubule Glutamylation, Dendritic Branching, and Neuronal MigrationASN NeuroJanuary 11, 2022Chen Liang, Damien Carrel, Nisha K. Singh, Liam L. Hiester, Isabelle Fanget, Hyuck Kim, Bonnie L. Firesteinglycerol, 0.5% Nonidet P40, 0.5% Triton X-100, and protease inhibitor cocktail [Cytoskeleton, Inc., cat# PIC02]), modified from a previous study (Audebert et al., 1993). Cells were homogenized, and the extract was

(4) A multiplexed phospholipid membrane platform for curvature sensitive protein screeningNanoscaleAugust 7, 2021Eider Berganza, Mirsana P. Ebrahimkutty, Srivatsan K. Vasantham, Chunting Zhong, Alexander Wunsch, Alexander Navarrete, Milos Galic, Michael Hirtzwere lysed with IP Lysis buﬀer (Pierce, 87787) supplemented with Protease inhibitors (Cytoskeleton, PIC02), followed by sedimentation on a table-top centrifuge (12 000 rpm). Only the supernatant was used for

(5) Ror2-mediated non-canonical Wnt signaling regulates Cdc42 and cell proliferation during tooth root developmentDevelopment (Cambridge, England)January 21, 2021Ma Y, Jing J, Feng J, Yuan Y, Wen Q, Han X, He J, Chen S, Ho TV, Chai Y.P3.5. Then tissues were lysed in lysis buffer including protease inhibitor cocktail (Cytoskeleton, PIC02) and protein fractions were isolated. According to the recommended protocol, GTP-loaded Cdc42 or Rac1

(6) Identification of berberine as a novel drug for the treatment of multiple myeloma via targeting UHRF1BMC BiologyDecember 1, 2020Chunming Gu, Zhao Yin, Hong Nie, Yanjun Liu, Juhua Yang, Guiping Huang, Jianping Shen, Liguo Chen, Jia Feiwith de-ubiquitinase inhibitor (part. #NEM09BB, Cytoskeleton) and protease inhibitor cocktail (cat. #PIC02, Cytoskeleton). Ubiquitinated proteins were immunoprecipitated using Signal-Seeker™ Ubiquitination Detection

(7) A Dynamic Variation of Pulmonary ACE2 Is Required to Modulate Neutrophilic Inflammation in Response to Pseudomonas aeruginosa Lung Infection in MiceThe Journal of ImmunologyDecember 1, 2019Chhinder P. Sodhi et al.BioProducts) containing phosphatase and protease inhibitor cocktail (#BP-480, Boston BioProducts, # PIC02, Cytoskeleton), and homogenized with homogenizing beads on a BeadBlaster (BenchMark) and centrifuged

(8) Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial ProteinsProteomesMay 22, 2018Horita H, Law A, Middleton K.with ice-cold BlastR lysis buffer containing a cocktail of TSA (1 µM), nicotinamide (16.5 mM), and PIC02, or NEM or Na3 VO4 . DNA was removed by passing the lysate through the BlastR filter system (Cytoskeleton,

(9) Actin polymerization contributes to enhanced pulmonary vasoconstrictor reactivity after chronic hypoxiaAmerican Journal of Physiology-Heart and Circulatory PhysiologyMay 1, 2018Laura Weise-Cross, Michelle A. Sands, Joshua R. Sheak, Brad R. S. Broughton, Jessica B. Snow, Laura V. Gonzalez Bosc, Nikki L. Jernigan, Benjimen R. Walker, Thomas C. Restaglass homogenizers in 200 ␮l of F-actin stabilization buffer supplemented with protease inhibitors (PIC02, Cytoskeleton) and phosphatase inhibitor cocktail (P0044, Sigma). After lysates had been cleared, 50

(10) Qiliqiangxin Rescues Mouse Cardiac Function by Regulating AGTR1/TRPV1-Mediated Autophagy in STZ-Induced Diabetes MellitusCellular Physiology and BiochemistryJanuary 1, 2018Jing Tong, Yan Lai, Yi-An Yao, Xue-Jun Wang, Yu-Shuang Shi, Han-Jin Hou, Jian-Yun Gu, Fei Chen, Xue-Bo LiumM sodium pyrophosphate, 1 mM phenylmethylsulfonyl fluoride, and protease inhibitor cocktail (Cat. # PIC02, Cytoskeleton), and equivalent amounts of protein were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis

(11) Activation of the Gi protein-RHOA axis by non-canonical Hedgehog signaling is independent of primary ciliaPloS oneJanuary 1, 2018Ho Wei L, Arastoo M, Georgiou I, Manning DR, Riobo-Del Galdo NA.(Santa Cruz Technology Dallas, TX), 0.5 μg/ml leupeptin, and 1X phosphate inhibitor cocktail (Catalog #PIC02, Cytoskeleton Inc., Denver, CO). Cell lysates for detection of GLI1 were prepared in 50 mM Tris-HCl

(12) Subcellular Localization and Activity of TRPM4 in Medial Prefrontal Cortex Layer 2/3Frontiers in cellular neuroscienceJanuary 1, 2018Riquelme D, Silva I, Philp AM, Huidobro-Toro JP, Cerda O, Trimmer JS, Leiva-Salcedo E.phenylmethylsulfonyl fluoride (PMSF; Sigma, 78830), and protease inhibitor cocktail (Cytoskeleton, Inc., PIC02) for 10 min at 4°C. The lysates were centrifuged at 12,000 g at 4°C for 10 min. The supernatants were

(13) Pulmonary Epithelial TLR4 Activation Leads to Lung Injury in Neonatal Necrotizing EnterocolitisJournal of immunology (Baltimore, Md. : 1950)August 1, 2016Jia H, Sodhi CP, Yamaguchi Y, Lu P, Martin LY, Good M, Zhou Q, Sung J, Fulton WB, Nino DF, et al.BioProducts) containing phosphatase and protease inhibitor cocktail (#BP-480, Boston BioProducts, # PIC02, Cytoskeleton), and homogenized with homogenizing beads on a BeadBlaster (BenchMark) and centrifuged

(14) The small GTPase RhoA, but not Rac1, is essential for conditioned aversive memory formation through regulation of actin rearrangements in rat dorsal hippocampusActa Pharmacologica SinicaJune 1, 2013Jun Wang, Yu-hua Wang, Yuan-yuan Hou, Tao Xi, Yao Liu, Jing-gen LiuStabilization Buffer (Cytoskeleton, Part# LAS01), with protease inhibitor Cocktail (1:100, Cytoskeleton, Cat # PIC02) and ATP (Cytoskeleton, Cat #BSA04-001), and then centrifuged at 37 °C at 100 000×g for 1 h. The G-actin

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Protease Inhibitor Cocktail (100x strength) from Cytoskeleton, Inc.

Cytoskeleton, Inc.

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Protease Inhibitor Cocktail (100x strength) from Cytoskeleton, Inc.

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